polyclonal rabbit anti psca antibody  (Boster Bio)

Journal: Scientific Reports

Article Title: PSCA rs2294008 polymorphism contributes to the decreased risk for cervical cancer in a Chinese population

doi: 10.1038/srep23465

Figure Lengend Snippet: Association between PSCA SNP rs2294008 and risk of cervical cancer.

Article Snippet: Series of paraffin sections of specimens were incubated with polyclonal rabbit anti-PSCA antibody (BA1694, BOSTER, Wuhan, China) overnight at 4 °C, and 3,3′-diaminobenzidine (DAB; Zhongshan Biotech, Beijing, China) was used to produce a brown precipitate.

Techniques:

Journal: Scientific Reports

Article Title: PSCA rs2294008 polymorphism contributes to the decreased risk for cervical cancer in a Chinese population

doi: 10.1038/srep23465

Figure Lengend Snippet: Upper panel, representative images were obtained at 400× magnification. The frequency distribution of the CC, CT, and TT genotypes of rs2294008 was 25, 19, and 6, respectively. Bottom, the histogram of the PSCA expression in each genotype. ** P < 0.01.

Article Snippet: Series of paraffin sections of specimens were incubated with polyclonal rabbit anti-PSCA antibody (BA1694, BOSTER, Wuhan, China) overnight at 4 °C, and 3,3′-diaminobenzidine (DAB; Zhongshan Biotech, Beijing, China) was used to produce a brown precipitate.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Positive and Negative Regulation of Prostate Stem Cell Antigen Expression by Yin Yang 1 in Prostate Epithelial Cell Lines

doi: 10.1371/journal.pone.0035570

Figure Lengend Snippet: A. Pten CaP8 and Pten CaP2 cells were incubated in hormone-depleted medium for 48 hours and then treated with 10 nM R1881 or vehicle (upper panels). Pten CaP2 and Pten CaP8 cells were infected with a lentivirus expressing YY1 specific siRNA or dsRed expression virus as control. Cells were then androgen deprived and treated with 10 nM R1881 for 48 hours (lower panels). Cell surface PSCA expression was determined by FACS using a polyclonal rabbit anti PSCA antibody. B. Expression of YY1 in Pten CaP8 and Pten CaP2 cells infected with YY1 siRNA expressing or control lentivirus was determined by Western blot. The blot was re-probed with anti-β-actin as a loading control. C. Quantitative real-time PCR analysis for PSCA expression was conducted on RNA extracted from Pten CaP2 cells in A.

Article Snippet: Cell surface PSCA protein levels were quantified by staining cell lines with rabbit polyclonal anti-PSCA antibody (0.2 μg/ml, Santa Cruz Biotechnology) followed by goat anti-rabbit-APC (1∶1000) and analyzed on a BD Facscalibur instrument using Cellquest Pro® software.

Techniques: Incubation, Infection, Expressing, Virus, Control, Western Blot, Real-time Polymerase Chain Reaction

Journal: PLoS ONE

Article Title: Positive and Negative Regulation of Prostate Stem Cell Antigen Expression by Yin Yang 1 in Prostate Epithelial Cell Lines

doi: 10.1371/journal.pone.0035570

Figure Lengend Snippet: A. LNCaP cells (2×10 5 /well) were infected in triplicate with YY1siRNA or scrambled control, and evaluated 72 hr later. PSCA message was determined by quantitative real-time PCR analysis. B. The amount of endogenous YY1 protein in YY1siRNA- or scramble control-infected cells was determined by Western blot. Representative analysis of one sample of triplicates is shown. C. PC-3 cells (2×10 5 /well) were infected in triplicate with YY1siRNA or scrambled control, and evaluated 72 hr later. PSCA message was determined by quantitative real-time PCR analysis. One independent repeat of the experiment was conducted with similar results. * = p<0.03. D. Endogenous YY1 protein in YY1siRNA- or scramble control-infected cells was determined by Western blot. Representative analysis of one sample of triplicates is shown.

Article Snippet: Cell surface PSCA protein levels were quantified by staining cell lines with rabbit polyclonal anti-PSCA antibody (0.2 μg/ml, Santa Cruz Biotechnology) followed by goat anti-rabbit-APC (1∶1000) and analyzed on a BD Facscalibur instrument using Cellquest Pro® software.

Techniques: Infection, Control, Real-time Polymerase Chain Reaction, Western Blot